Glycosylation Analysis Service for Chikungunya Virus Glycoprotein

Chikungunya Virus Glycoprotein

Chikungunya virus (CHIKV) is classified within the Togaviridae family, Alphavirus genus, and is characterized as an enveloped positive-sense RNA virus. CHIKV invades susceptible cells through two structural glycoproteins, E2 and E1, which are crucial for viral attachment and membrane fusion. These glycoprotein heterodimers are integrated into the viral membrane and carry the primary antigenic determinants, making them the key targets for eliciting a neutralizing antibody response. Sequence analysis has identified that E1 possesses a single N-linked glycosylation site at N141, and E2 has two glycosylation sites located at N263 and N345. N-linked glycans have demonstrated their significance in alphavirus biology, influencing host interferon responses and tropism modulation. Earlier studies have shown that CHIKV strains lacking one or both of the N-linked glycans on E2 displayed a significant reduction in their ability to induce inflammation in infected mice. Therefore, a comprehensive glycosylation profiling of CHIKV glycoproteins is essential for understanding their biological properties and can provide valuable insights for the development of CHIKV vaccines.

Fig.1 Structure of CHIKV.¹

Glycosylation Analysis Services for CHIKV Glycoprotein at Creative Biolabs

Creative Biolabs leads the way in glycosylation analysis services for viral glycoproteins. Our pioneering expertise, coupled with cutting-edge methodologies, has enabled us to establish an innovative platform for glycosylation profiling tailored for CHIKV across various host species. We harness tandem mass spectrometry to characterize N-glycosylation patterns on CHIKV glycoproteins. This characterization includes individual site occupancy status (macroheterogeneity) and site-specific structural diversity (microheterogeneity) of CHIKV E1 and E2. Our mass spectrometry-based analysis of viral glycans serves as a powerful tool, unveiling not only the heterogeneity of N-glycan structures present within a single protein but also facilitating meaningful comparisons of the distinct glycosylation patterns of CHIKV in various host species.

Applications of Viral Glycosylation Analysis: CHIKV Vaccine Development

Glycosylation analysis of CHIKV glycoproteins is indispensable for vaccine development. Various vaccine strategies have been explored for CHIKV, such as virus-like particle (VLP) -based vaccines and subunit vaccines (CHIKV E1, E2). It's important to note that vaccine antigens expressed in different host cells may exhibit significantly different N-glycosylation patterns.

Our glycosylation analysis services play a pivotal role in selecting an appropriate expression platform for vaccine manufacturing and facilitating glycosylation immunogenicity correlation studies. These studies enable a comprehensive evaluation of how these glycan variations influence vaccine immunogenicity. Furthermore, continuous monitoring of glycosylation throughout the various stages of process development is essential to ensure the consistency, safety, and efficacy of vaccine products.

Benefits of Our Services

• Utilization of high-resolution MS-based detection techniques
• Thorough and comprehensive profiling of N-glycans
• Systematic comparison of glycosylation patterns in different host cells
• A dedicated team of experts with extensive experience in glycoprotein analysis

Creative Biolabs has developed state-of-the-art MS-based analysis platforms tailored for CHIKV E1 and E2 glycoproteins. These advanced platforms empower us to delve into the intricate N-glycan patterns at each glycosylation site and this in-depth analysis plays a pivotal role in propelling vaccine development efforts forward.

If you have specific questions regarding our viral glycoprotein analysis services, please feel free to contact us. Our dedicated team is readily available to provide the expertise and guidance you require for your research endeavors.

Reference

1. Castillo-Macías, A., et al. "Immunology of viral infections with a high impact in Mexico: Dengue, Chikungunya, and Zika." Medicina Universitaria 19.77 (2017): 198-207.

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