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Quantitative Canine Anti-β2 Glycoprotein Antibody Competitive ELISA Kit

CAT#: GLJF-0225-HX54

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Specifications
Product Description
The quantitative canine anti-β2 glycoprotein antibody competitive ELISA kit is designed to detect the level of canine anti-β2 glycoprotein antibody (β2GPI-Ab). Employing the principles of enzyme-linked immunosorbent assay, the kit leverages the specific antigen-antibody interaction, followed by an enzyme-catalyzed colorimetric reaction. The resulting absorbance is then measured to determine the antibody concentration. Compatible with a wide range of biological samples, including tissue homogenates, cell culture supernatants, serum, or plasma, this kit offers high sensitivity and specificity, enabling the precise detection of even low concentrations of anti-β2 glycoprotein antibodies.
Target
β2GPI-Ab
Sample Types
Tissue homogenates, cell culture supernatants, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
2 ng/mL
Detection Principle
Quantitative competitive ELISA
Detection Range
25 ng/mL-200 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Canine
Full Name
Anti-β2 glycoprotein antibody
Alternate Names
Anti-β2-GP1 antibody; Anti-β2 glycoprotein type I antibody; β2-GPI antibody; A-B2GA; Anti-beta-2-glycoprotein 1 antibody; Anti-beta-2-GPI antibody; β2GP1 antibody; β2GPI-Ab
Application Notes
Application
Anti-β2-glycoprotein antibody is an autoantibody against β2-glycoprotein I (β2-GPI), a plasma protein that plays an important role in the coagulation process. The presence of this antibody is closely related to autoimmune diseases such as antiphospholipid syndrome (APS). The quantitative canine anti-β2 glycoprotein antibody competitive ELISA kit is used to detect anti-β2-GPI antibodies in biological samples, which can be used to study the pathogenesis of autoimmune diseases and provide an important experimental means for in-depth understanding of disease pathology and development of new therapies.
Kit Components
Pre-coated 96-well ELISA plate; Standard solution; Enzyme conjugate; Various diluents; Wash buffer; Substrate solution; Stop solution
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
92-105%
For Research Use Only.
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