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Quantitative Human βACE1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX480

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Specifications
Product Description
The quantitative human βACE1 (glycosylated) sandwich ELISA kit is designed to detect human β-site APP cleaving enzyme 1 (βACE1) levels. BACE1 is an enzyme that plays a crucial role in the production of amyloid-beta peptides. Amyloid-beta peptides are involved in the development of Alzheimer's disease. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of βACE1 in the sample.
Target
βACE1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.128 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
β-Site APP cleaving enzyme 1
Alternate Names
βACE1; β-Site APP cleaving enzyme 1; ASP2; Memapsin 2; Aspartyl protease 2; Membrane-associated aspartic protease 2
Uniprot No.
N-Glycosylation Site
153, 172, 223, 354
Application Notes
Application
The quantitative human βACE1 (glycosylated) sandwich ELISA kit is used to measure glycosylated BACE1 levels in samples. This measurement is important in research related to Alzheimer's disease. The kit is applied in studies of the disease process, which helps in understanding the role of BACE1.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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