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Quantitative Human βACE1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX480)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human βACE1 (glycosylated) sandwich ELISA kit is designed to detect human β-site APP cleaving enzyme 1 (βACE1) levels. BACE1 is an enzyme that plays a crucial role in the production of amyloid-beta peptides. Amyloid-beta peptides are involved in the development of Alzheimer's disease. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of βACE1 in the sample.
Target βACE1
N-Glycosylation Site 153, 172, 223, 354
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.128 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name β-Site APP cleaving enzyme 1
Alternate Names βACE1; β-Site APP cleaving enzyme 1; ASP2; Memapsin 2; Aspartyl protease 2; Membrane-associated aspartic protease 2
Uniprot No. P56817
Application The quantitative human βACE1 (glycosylated) sandwich ELISA kit is used to measure glycosylated BACE1 levels in samples. This measurement is important in research related to Alzheimer's disease. The kit is applied in studies of the disease process, which helps in understanding the role of BACE1.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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