Quantitative Human APOL1 (Glycosylated) Sandwich ELISA Kit

The quantitative human APOL1 (glycosylated) sandwich ELISA kit is designed to detect human apolipoprotein L (APOL1) levels. APOL1 is a protein involved in lipid transport and innate immunity, particularly against Trypanosoma brucei parasites. It plays a role in various physiological processes, including lipid metabolism and cell death. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.792 ng/mL, which can accurately detect low concentrations of APOL1 in the sample.

APOL1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.792 ng/mL

Quantitative sandwich ELISA

2 ng/mL-75 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Apolipoprotein L

APOL1; Apolipoprotein L; Apo-L; APOL-I

261

The quantitative human APOL1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating kidney diseases, particularly focal segmental glomerulosclerosis (FSGS) and hypertensive nephropathy, where APOL1 genetic variants are associated with increased risk. By quantifying APOL1, researchers can gain insights into its role in disease pathogenesis and explore potential diagnostic or therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.