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Quantitative Human APOL1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX30)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human APOL1 (glycosylated) sandwich ELISA kit is designed to detect human apolipoprotein L (APOL1) levels. APOL1 is a protein involved in lipid transport and innate immunity, particularly against Trypanosoma brucei parasites. It plays a role in various physiological processes, including lipid metabolism and cell death. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.792 ng/mL, which can accurately detect low concentrations of APOL1 in the sample.
Target APOL1
N-Glycosylation Site 261
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.792 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-75 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Apolipoprotein L
Alternate Names APOL1; Apolipoprotein L; Apo-L; APOL-I
Uniprot No. O14791
Application The quantitative human APOL1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating kidney diseases, particularly focal segmental glomerulosclerosis (FSGS) and hypertensive nephropathy, where APOL1 genetic variants are associated with increased risk. By quantifying APOL1, researchers can gain insights into its role in disease pathogenesis and explore potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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