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Quantitative Human ASAH2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX840)

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1×48 T
1×96 T

Product Description The quantitative human ASAH2 (glycosylated) sandwich ELISA kit is designed to detect human N-Acylsphingosine amidohydrolase 2 (ASAH2) levels. ASAH2 is an enzyme that catalyzes the hydrolysis of ceramides. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.524 ng/mL, which can accurately detect low concentrations of ASAH2 in the sample.
Target ASAH2
N-Glycosylation Site 98, 151, 217, 308, 440, 468, 564, 730
O-Glycosylation Site 62, 67, 68, 70, 73, 74, 76, 78, 79, 80, 82, 84, 779
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.524 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.25 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name N-Acylsphingosine amidohydrolase 2
Alternate Names ASAH2; N-Acylsphingosine amidohydrolase 2; HNAC1; Neutral ceramidase
Uniprot No. Q9NR71
Application The quantitative human ASAH2 (glycosylated) sandwich ELISA kit is used to determine ASAH2 levels in biological samples. This kit is applicable in studies examining ceramide metabolism and metabolic diseases. It is also relevant to studies investigating the role of ASAH2 in cellular signaling pathways and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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