Quantitative Human ASAH2 (Glycosylated) Sandwich ELISA Kit

The quantitative human ASAH2 (glycosylated) sandwich ELISA kit is designed to detect human N-Acylsphingosine amidohydrolase 2 (ASAH2) levels. ASAH2 is an enzyme that catalyzes the hydrolysis of ceramides. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.524 ng/mL, which can accurately detect low concentrations of ASAH2 in the sample.

ASAH2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.524 ng/mL

Quantitative sandwich ELISA

1.25 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

N-Acylsphingosine amidohydrolase 2

ASAH2; N-Acylsphingosine amidohydrolase 2; HNAC1; Neutral ceramidase

98, 151, 217, 308, 440, 468, 564, 730

62, 67, 68, 70, 73, 74, 76, 78, 79, 80, 82, 84, 779

The quantitative human ASAH2 (glycosylated) sandwich ELISA kit is used to determine ASAH2 levels in biological samples. This kit is applicable in studies examining ceramide metabolism and metabolic diseases. It is also relevant to studies investigating the role of ASAH2 in cellular signaling pathways and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.