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Quantitative Human BCAN (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX735

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Specifications
Product Description
The quantitative human BCAN (glycosylated) sandwich ELISA kit is designed to detect human brevican (BCAN) levels. BCAN is an extracellular matrix protein that plays a role in the formation of perineuronal nets. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.134 ng/mL, which can accurately detect low concentrations of BCAN in the sample.
Target
BCAN
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.134 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Brevican
Alternate Names
BCAN; Brevican; BEHAB; CSPG7; Chondroitin sulfate proteoglycan 7; Brevican core protein; Brain-enriched hyaluronan-binding protein
Uniprot No.
N-Glycosylation Site
130, 337
O-Glycosylation Site
418, 905, 906
Application Notes
Application
The quantitative human BCAN (glycosylated) sandwich ELISA kit provides a method for quantifying glycosylated BCAN in biological samples. This measurement is valuable for research on brain structure and extracellular matrix. The kit also has potential applications in investigations of neurological disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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