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Quantitative Human C5aR1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX300)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human C5aR1 (glycosylated) sandwich ELISA kit is designed to detect human complement component 5A receptor 1 (C5aR1) levels. C5aR1 protein is a crucial G protein-coupled receptor. This receptor is highly expressed on various immune cells, including neutrophils, monocytes, and macrophages. C5aR1 plays a significant role in the inflammatory response by binding to the complement component C5a. This interaction triggers a cascade of intracellular signaling events, leading to cellular activation, chemotaxis, and the release of inflammatory mediators. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.138 ng/mL, which can accurately detect low concentrations of C5aR1 in the sample.
Target C5aR1
N-Glycosylation Site 5
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.138 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Complement component 5A receptor 1
Alternate Names C5aR1; Complement component 5A receptor 1; CD88; C5A; C5R1
Uniprot No. P21730
Application The quantitative human C5aR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated C5aR1 levels in biological samples. Researchers utilize this tool to investigate the role of C5aR1 in various physiological and pathological processes. It finds applications in studies related to inflammation, autoimmune diseases, and infectious diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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