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Quantitative Human COL11α2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX242

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Specifications
Product Description
The quantitative human COL11α2 (glycosylated) sandwich ELISA kit is designed to detect human collagen type XI α2 (COL11α2) levels. Collagen type XI α2 is a protein encoded by the COL11A2 gene, and it forms a component of type XI collagen, which plays a crucial role in the structural integrity of cartilage and other connective tissues. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of COL11α2 in the sample.
Target
COL11α2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.091 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Collagen type XI α2
Alternate Names
COL11α2; Collagen type XI α2; COL11-A2; DFNA13; Collagen α2(XI) chain
Uniprot No.
N-Glycosylation Site
1604
Application Notes
Application
The quantitative human COL11α2 (glycosylated) sandwich ELISA kit enables researchers to quantify this specific collagen isoform, aiding in studies related to cartilage metabolism, connective tissue disorders, and related research areas. By utilizing a sandwich ELISA format, it provides a sensitive and reliable method for detecting and quantifying COL11α2.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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