Quantitative Human CYP1A1 (Glycosylated) Sandwich ELISA Kit

The quantitative human CYP1A1 (glycosylated) sandwich ELISA kit is designed to detect human cytochrome P450 1A1 (CYP1A1) levels. CYP1A1 is an enzyme involved in the metabolism of various xenobiotics, including polycyclic aromatic hydrocarbons (PAHs), playing a role in both detoxification and activation of procarcinogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.102 ng/mL, which can accurately detect low concentrations of CYP1A1 in the sample.

CYP1A1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.102 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cytochrome P450 1A1

CYP1A1; Cytochrome P450 1A1; CYP1; AHRR; P1-450

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The quantitative human CYP1A1 (glycosylated) sandwich ELISA kit is valuable in research investigating environmental toxicology, drug metabolism, and cancer, where CYP1A1 expression and activity are relevant. Researchers employ this ELISA to quantify CYP1A1 levels, providing insights into its role in xenobiotic metabolism and potential health risks.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.