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Quantitative Human CYP1A1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX172)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CYP1A1 (glycosylated) sandwich ELISA kit is designed to detect human cytochrome P450 1A1 (CYP1A1) levels. CYP1A1 is an enzyme involved in the metabolism of various xenobiotics, including polycyclic aromatic hydrocarbons (PAHs), playing a role in both detoxification and activation of procarcinogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.102 ng/mL, which can accurately detect low concentrations of CYP1A1 in the sample.
Target CYP1A1
O-Glycosylation Site 67
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.102 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cytochrome P450 1A1
Alternate Names CYP1A1; Cytochrome P450 1A1; CYP1; AHRR; P1-450
Uniprot No. P04798
Application The quantitative human CYP1A1 (glycosylated) sandwich ELISA kit is valuable in research investigating environmental toxicology, drug metabolism, and cancer, where CYP1A1 expression and activity are relevant. Researchers employ this ELISA to quantify CYP1A1 levels, providing insights into its role in xenobiotic metabolism and potential health risks.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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