Quantitative Human DAT (Glycosylated) Sandwich ELISA Kit

The quantitative human DAT (glycosylated) sandwich ELISA kit is designed to detect human dopamine transporter (DAT) levels. DAT is a membrane protein that plays a critical role in regulating dopamine neurotransmission. DAT is located on the presynaptic neuron and functions to remove dopamine from the synaptic cleft, the space between neurons. This reuptake process terminates dopamine signaling and allows for its recycling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of DAT in the sample.

DAT

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.083 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Dopamine transporter

DAT; Dopamine transporter; SLC6A3; DAT1; Sodium-dependent dopamine transporter; Dopamine active transporter

181, 188, 205

The quantitative human DAT (glycosylated) sandwich ELISA kit is a valuable tool for neuroscience research, particularly in studies of dopamine signaling and related disorders. Researchers use it to measure DAT levels in brain tissue and other biological samples. The kit has applications in studies of Parkinson's disease, addiction, and attention deficit hyperactivity disorder (ADHD).

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.