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Quantitative Human DAT (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX516)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human DAT (glycosylated) sandwich ELISA kit is designed to detect human dopamine transporter (DAT) levels. DAT is a membrane protein that plays a critical role in regulating dopamine neurotransmission. DAT is located on the presynaptic neuron and functions to remove dopamine from the synaptic cleft, the space between neurons. This reuptake process terminates dopamine signaling and allows for its recycling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of DAT in the sample.
Target DAT
N-Glycosylation Site 181, 188, 205
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.083 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Dopamine transporter
Alternate Names DAT; Dopamine transporter; SLC6A3; DAT1; Sodium-dependent dopamine transporter; Dopamine active transporter
Uniprot No. Q01959
Application The quantitative human DAT (glycosylated) sandwich ELISA kit is a valuable tool for neuroscience research, particularly in studies of dopamine signaling and related disorders. Researchers use it to measure DAT levels in brain tissue and other biological samples. The kit has applications in studies of Parkinson's disease, addiction, and attention deficit hyperactivity disorder (ADHD).
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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