Quantitative Human DPAGT1 (Glycosylated) Sandwich ELISA Kit

The quantitative human DPAGT1 (glycosylated) sandwich ELISA kit is designed to detect human dolichyl phosphate-N-acetylglucosaminephosphotransferase 1 (DPAGT1) levels. DPAGT1 is an enzyme that is located in the endoplasmic reticulum membrane. DPAGT1 catalyzes the first step in the glycosylation pathway. This enzyme is essential for the synthesis of N-glycans, which are crucial for protein folding and functions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of DPAGT1 in the sample.

DPAGT1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.093 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Dolichyl phosphate-N-acetylglucosaminephosphotransferase 1

DPAGT1; Dolichyl phosphate-N-acetylglucosaminephosphotransferase 1; DGPT; DPAGT; G1PT; UAGT; GlcNAc-1-P transferase

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The quantitative human DPAGT1 (glycosylated) sandwich ELISA kit provides a means to quantitatively measure DPAGT1 levels in biological samples. It is valuable for studies examining protein glycosylation. This kit has applications in research focused on congenital disorders of glycosylation. It is also relevant to studies investigating the role of glycosylation in various cellular processes.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.