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Quantitative Human ERAP2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX642)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ERAP2 (glycosylated) sandwich ELISA kit is designed to detect human endoplasmic reticulum aminopeptidase 2 (ERAP2) levels. ERAP2 is an enzyme that trims peptides in the endoplasmic reticulum (ER). This trimming process is crucial for the presentation of antigenic peptides by MHC class I molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of ERAP2 in the sample.
Target ERAP2
N-Glycosylation Site 85, 119, 219, 405, 650
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.140 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Endoplasmic reticulum aminopeptidase 2
Alternate Names ERAP2; Endoplasmic reticulum aminopeptidase 2; LRAP; Leukocyte-derived arginine aminopeptidase
Uniprot No. Q6P179
Application The quantitative human ERAP2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ERAP2 levels in biological samples. The kit is applicable in studies of antigen processing, immune responses, and MHC class I presentation. It is useful in fields such as immunology and cell biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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