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Quantitative Human ERAP2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX642

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Specifications
Product Description
The quantitative human ERAP2 (glycosylated) sandwich ELISA kit is designed to detect human endoplasmic reticulum aminopeptidase 2 (ERAP2) levels. ERAP2 is an enzyme that trims peptides in the endoplasmic reticulum (ER). This trimming process is crucial for the presentation of antigenic peptides by MHC class I molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of ERAP2 in the sample.
Target
ERAP2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.140 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Endoplasmic reticulum aminopeptidase 2
Alternate Names
ERAP2; Endoplasmic reticulum aminopeptidase 2; LRAP; Leukocyte-derived arginine aminopeptidase
Uniprot No.
N-Glycosylation Site
85, 119, 219, 405, 650
Application Notes
Application
The quantitative human ERAP2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ERAP2 levels in biological samples. The kit is applicable in studies of antigen processing, immune responses, and MHC class I presentation. It is useful in fields such as immunology and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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