Quantitative Human EXT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX613)

Name: Quantitative Human EXT1 (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX613
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human EXT1 (glycosylated) sandwich ELISA kit is designed to detect human exostoses 1 (EXT1) levels. EXT1 is a tumor suppressor gene that encodes a glycosyltransferase enzyme. This enzyme is involved in the synthesis of heparan sulfate, a complex carbohydrate found on the cell surface and in the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.359 ng/mL, which can accurately detect low concentrations of EXT1 in the sample.
Target	EXT1
N-Glycosylation Site	89, 330
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.359 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1.5 ng/mL-45 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Application	The quantitative human EXT1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EXT1 levels in biological samples. The kit is applicable in studies of glycosaminoglycan synthesis, tumor suppression, and bone development. It is useful in fields such as cell biology and oncology.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 85-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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