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Quantitative Human EXT1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX613

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Specifications
Product Description
The quantitative human EXT1 (glycosylated) sandwich ELISA kit is designed to detect human exostoses 1 (EXT1) levels. EXT1 is a tumor suppressor gene that encodes a glycosyltransferase enzyme. This enzyme is involved in the synthesis of heparan sulfate, a complex carbohydrate found on the cell surface and in the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.359 ng/mL, which can accurately detect low concentrations of EXT1 in the sample.
Target
EXT1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.359 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.5 ng/mL-45 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Exostoses 1
Alternate Names
EXT1; Exostoses 1; LGCR, LGS
Uniprot No.
N-Glycosylation Site
89, 330
Application Notes
Application
The quantitative human EXT1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EXT1 levels in biological samples. The kit is applicable in studies of glycosaminoglycan synthesis, tumor suppression, and bone development. It is useful in fields such as cell biology and oncology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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