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Quantitative Human Flt3L (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX441

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Specifications
Product Description
The quantitative human Flt3L (glycosylated) sandwich ELISA kit is designed to detect human FMS like tyrosine kinase 3 ligand (Flt3L) levels. Flt3L is a cytokine that stimulates the growth and differentiation of hematopoietic stem cells, which plays a role in the development of various immune cells, including dendritic cells. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 12.250 pg/mL, which can accurately detect low concentrations of Flt3L in the sample.
Target
Flt3L
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
12.250 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
FMS like tyrosine kinase 3 ligand
Alternate Names
Flt3L; FMS like tyrosine kinase 3 ligand; FLT3-LG; FL; Fms-related tyrosine kinase 3 ligand
Uniprot No.
N-Glycosylation Site
126, 149
Application Notes
Application
The quantitative human Flt3L (glycosylated) sandwich ELISA kit is used to measure glycosylated Flt3L levels in samples. This measurement is important in research related to hematopoiesis and immunology. The kit is applied in studies of conditions affecting immune cell development, which helps in understanding the role of Flt3L.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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