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Quantitative Human HRH1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX383

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Specifications
Product Description
The quantitative human HRH1 (glycosylated) sandwich ELISA kit is designed to detect human histamine receptor H1 (HRH1) levels. HRH1 is a G protein-coupled receptor, which binds to histamine. HRH1 plays a role in mediating allergic reactions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of HRH1 in the sample.
Target
HRH1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.120 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Histamine receptor H1
Alternate Names
HRH1; Histamine receptor H1
Uniprot No.
N-Glycosylation Site
5, 18
Application Notes
Application
The quantitative human HRH1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HRH1 levels. It is useful for studying the role of histamine in allergic responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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