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Quantitative Human IFI30 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX236)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IFI30 (glycosylated) sandwich ELISA kit is designed to detect human interferon γ inducible protein 30 (IFI30) levels. IFI30 is a lysosomal thiol reductase involved in antigen processing and presentation. It plays a role in generating peptides for MHC class II loading. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.075 ng/mL, which can accurately detect low concentrations of IFI30 in the sample.
Target IFI30
N-Glycosylation Site 63, 95, 108
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.075 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Interferon γ inducible protein 30
Alternate Names IFI30; Interferon γ inducible protein 30; GILT; IP30; γ-Interferon-inducible lysosomal thiol reductase
Uniprot No. P13284
Application The quantitative human IFI30 (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly antigen presentation and autoimmune diseases. Researchers utilize this ELISA to quantify IFI30 levels, providing insights into its role in immune regulation and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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