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Quantitative Human IFI30 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX236

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Specifications
Product Description
The quantitative human IFI30 (glycosylated) sandwich ELISA kit is designed to detect human interferon γ inducible protein 30 (IFI30) levels. IFI30 is a lysosomal thiol reductase involved in antigen processing and presentation. It plays a role in generating peptides for MHC class II loading. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.075 ng/mL, which can accurately detect low concentrations of IFI30 in the sample.
Target
IFI30
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.075 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interferon γ inducible protein 30
Alternate Names
IFI30; Interferon γ inducible protein 30; GILT; IP30; γ-Interferon-inducible lysosomal thiol reductase
Uniprot No.
N-Glycosylation Site
63, 95, 108
Application Notes
Application
The quantitative human IFI30 (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly antigen presentation and autoimmune diseases. Researchers utilize this ELISA to quantify IFI30 levels, providing insights into its role in immune regulation and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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