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Quantitative Human IFNα2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX137

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Specifications
Product Description
The quantitative human IFNα2 (glycosylated) sandwich ELISA kit is designed to detect human interferon α2 (IFNα2) levels. IFNα2 is a type I interferon, a cytokine that plays a crucial role in antiviral immunity and immune regulation. It is commonly used in the treatment of viral infections and certain cancers. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 8.271 pg/mL, which can accurately detect low concentrations of IFNα2 in the sample.
Target
IFNα2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
8.271 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interferon α2
Alternate Names
IFNα2; Interferon α2; IFNα2A; IFNα2B; α2a Interferon; Interferon α2b; Interferon αA
Uniprot No.
O-Glycosylation Site
129
Application Notes
Application
The quantitative human IFNα2 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for monitoring IFNα2 levels during therapeutic interventions and for investigating its role in immune responses and disease pathogenesis. Researchers use this ELISA to quantify IFNα2, providing insights into its efficacy and potential side effects in various medical applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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