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Quantitative Human IL21 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX822

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Specifications
Product Description
The quantitative human IL21 (glycosylated) sandwich ELISA kit is designed to detect human interleukin 21 (IL21) levels. IL21 is a cytokine belonging to the IL-15/IL-21 family that is produced by activated T cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 7.120 pg/mL, which can accurately detect low concentrations of IL21 in the sample.
Target
IL21
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
7.120 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
22.25 pg/mL-950 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 21
Alternate Names
IL21; Interleukin 21
Uniprot No.
N-Glycosylation Site
97
Application Notes
Application
The quantitative human IL21 (glycosylated) sandwich ELISA kit is used to measure IL21 levels in biological samples. This kit is applicable in studies examining cytokine signaling and autoimmune diseases. It is also relevant to studies investigating the role of IL21 in regulating immune cell functions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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