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Quantitative Human LOXL3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX484

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Specifications
Product Description
The quantitative human LOXL3 (glycosylated) sandwich ELISA kit is designed to detect human lysyl oxidase like protein 3 (LOXL3) levels. LOXL3 is an enzyme that belongs to the lysyl oxidase family. Lysyl oxidases are involved in the crosslinking of collagen and elastin. LOXL3 may play a role in the structural integrity of the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of LOXL3 in the sample.
Target
LOXL3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lysyl oxidase like protein 3
Alternate Names
LOXL3; Lysyl oxidase like protein 3; LOXL; Lysyl oxidase homolog 3
Uniprot No.
N-Glycosylation Site
111, 266, 390, 481, 625
Application Notes
Application
The quantitative human LOXL3 (glycosylated) sandwich ELISA kit is used to measure glycosylated LOXL3 levels in samples. This measurement is important in research related to extracellular matrix biology. The kit is applied in studies of tissue structure, which helps in understanding the role of LOXL3.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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