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Quantitative Human MHCF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX359

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Specifications
Product Description
The quantitative human MHCF (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 1F (MHCF) levels. MHCF is a component of cardiac muscle, which plays a role in heart muscle contraction. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 5.863 pg/mL, which can accurately detect low concentrations of MHCF in the sample.
Target
MHCF
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
5.863 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Major histocompatibility complex class 1F
Alternate Names
MHCF; Major histocompatibility complex class 1F; CDA12; HLA F antigen; HLAF; MHC class I antigen F
Uniprot No.
N-Glycosylation Site
107
Application Notes
Application
The quantitative human MHCF (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated MHCF levels. It is useful for studying the role of MHCF in cardiac muscle functions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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