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Quantitative Human NIS (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX723

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Specifications
Product Description
The quantitative human NIS (glycosylated) sandwich ELISA kit is designed to detect human sodium iodide symporter (NIS) levels. NIS is a membrane protein, which transports iodide into thyroid cells. NIS is essential for thyroid hormone synthesis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.064 ng/mL, which can accurately detect low concentrations of NIS in the sample.
Target
NIS
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.064 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sodium iodide symporter
Alternate Names
NIS; Sodium iodide symporter; SLC5A5; Solute carrier family 5 member 5
Uniprot No.
N-Glycosylation Site
489, 502
Application Notes
Application
The quantitative human NIS (glycosylated) sandwich ELISA kit is useful for measuring NIS levels in samples. It can be applied in studies of thyroid functions and thyroid diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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