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Quantitative Human PAM (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX274

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Specifications
Product Description
The quantitative human PAM (glycosylated) sandwich ELISA kit is designed to detect human peptidylglycine α amidating monooxygenase (PAM) levels. PAM is an enzyme that catalyzes the formation of alpha-amidated peptides. This protein is involved in the synthesis of many peptide hormones, which plays a role in neurotransmission and endocrine functions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 15.028 pg/mL, which can accurately detect low concentrations of PAM in the sample.
Target
PAM
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
15.028 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
35 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Peptidylglycine α amidating monooxygenase
Alternate Names
PAM; Peptidylglycine α amidating monooxygenase; PAL; PHM
Uniprot No.
N-Glycosylation Site
762
Application Notes
Application
The quantitative human PAM (glycosylated) sandwich ELISA kit provides a tool for quantitatively measuring glycosylated PAM levels in biological samples. It can be used in studies investigating hormone processing, neurological disorders, and cancer. The kit is valuable in endocrinology research.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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