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Quantitative Human PAM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX274)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PAM (glycosylated) sandwich ELISA kit is designed to detect human peptidylglycine α amidating monooxygenase (PAM) levels. PAM is an enzyme that catalyzes the formation of alpha-amidated peptides. This protein is involved in the synthesis of many peptide hormones, which plays a role in neurotransmission and endocrine functions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 15.028 pg/mL, which can accurately detect low concentrations of PAM in the sample.
Target PAM
N-Glycosylation Site 762
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 15.028 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 35 pg/mL-1500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Peptidylglycine α amidating monooxygenase
Alternate Names PAM; Peptidylglycine α amidating monooxygenase; PAL; PHM
Uniprot No. P19021
Application The quantitative human PAM (glycosylated) sandwich ELISA kit provides a tool for quantitatively measuring glycosylated PAM levels in biological samples. It can be used in studies investigating hormone processing, neurological disorders, and cancer. The kit is valuable in endocrinology research.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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