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Quantitative Human PLOD2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX16

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Specifications
Product Description
The quantitative human PLOD2 (glycosylated) sandwich ELISA kit is designed to detect human procollagen lysine-2-oxoglutarate-5-dioxygenase 2 (PLOD2) levels. PLOD2 is an enzyme that hydroxylates lysine residues in collagen, playing a crucial role in collagen crosslinking and extracellular matrix remodeling. It is involved in various physiological processes, including tissue development, wound healing, and fibrosis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of PLOD2 in the sample.
Target
PLOD2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Procollagen lysine-2-oxoglutarate-5-dioxygenase 2
Alternate Names
PLOD2; Procollagen lysine-2-oxoglutarate-5-dioxygenase 2; Telopeptide lysyl hydroxylase; Lysyl hydroxylase 2; TLH; LH2
Uniprot No.
N-Glycosylation Site
63, 209, 297, 365, 522, 696, 725
Application Notes
Application
The quantitative human PLOD2 (glycosylated) sandwich ELISA kit is useful for researchers studying fibrosis, cancer, and skeletal disorders, where PLOD2 activity is often dysregulated. By quantifying PLOD2, researchers can investigate its contribution to disease pathogenesis and explore potential therapeutic targets related to extracellular matrix alterations.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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