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Quantitative Human PSMα7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX31

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Specifications
Product Description
The quantitative human PSMα7 (glycosylated) sandwich ELISA kit is designed to detect human proteasome subunit α type 7 (PSMα7) levels. PSMα7 is a core component of the 20S proteasome, a multi-subunit protease complex responsible for intracellular protein degradation. It plays a critical role in cellular homeostasis by regulating protein turnover. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.127 ng/mL, which can accurately detect low concentrations of PSMα7 in the sample.
Target
PSMα7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.127 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Proteasome subunit α type 7
Alternate Names
PSMα7; Proteasome subunit α type 7
Uniprot No.
O-Glycosylation Site
130
Application Notes
Application
The quantitative human PSMα7 (glycosylated) sandwich ELISA kit is useful for researchers investigating cancer, neurodegenerative diseases, and inflammatory conditions, where proteasome function is often dysregulated. By quantifying PSMα7, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to proteasome modulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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