Quantitative Human RSPO3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX787)

Product Description	The quantitative human RSPO3 (glycosylated) sandwich ELISA kit is designed to detect human r-spondin 3 (RSPO3) levels. RSPO3 is a secreted protein that modulates Wnt signaling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of RSPO3 in the sample.
Target	RSPO3
N-Glycosylation Site	36
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.128 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	R-Spondin 3
Alternate Names	RSPO3; R-Spondin 3; PWTSR; THSD2; Roof plate-specific spondin-3
Uniprot No.	Q9BXY4

Application	The quantitative human RSPO3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated RSPO3 in samples. This quantification is important for research on Wnt signaling and developmental biology. The kit also has potential applications in investigations of cancer.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 85-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.