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Quantitative Human WNT3A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1224

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human WNT3A (glycosylated) sandwich ELISA kit is designed to detect human wingless-type MMTV integration site family, member 3A (WNT3A) levels. It is suitable for various biological samples such as serum or plasma. Its sensitivity is 0.047 ng/mL, which can accurately detect low concentrations of WNT3A in the sample.
Target
WNT3A
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated WNT3A antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.047 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.16 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless-type MMTV integration site family, member 3A
Alternate Names
Wingless-type MMTV integration site family, member 3A; Protein Wnt 3A precursor; Wnt-3a; WNT3A
Uniprot No.
P56704
N-Glycosylation Site
87, 298
Application Notes
Application
The quantitative human WNT3A (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of WNT3A, helping to understand its role in stem cells.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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