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Quantitative Rat C3 (Glycosylated) Competitive ELISA Kit

CAT#: GLJF-0325-HX76

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative rat C3 (glycosylated) competitive ELISA kit is designed to detect rat complement 3 (C3) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.162 μg/mL, which can accurately detect low concentrations of C3 in the sample.
Target
C3
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; HRP-conjugated C3 antibody; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.162 μg/mL
Detection Principle
Quantitative competitive ELISA
Detection Range
0.65 μg/mL-40 μg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Complement 3
Alternate Names
Complement C3 α chain; C3a anaphylatoxin; Acylation stimulating protein; ASP; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; C3
Uniprot No.
P01026
N-Glycosylation Site
939, 1617
Application Notes
Application
The quantitative rat C3 (glycosylated) competitive ELISA kit is widely used to assess the expression levels of C3, helping to understand its role in cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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