Flavonol 3-O-glucosyltransferase plays a pivotal role in the biosynthesis of flavonol 3-O-β-D-glucosyl-(1→2)-β-D-glucoside, a valuable compound with diverse biological activities. This Glycan Tool Enzyme catalyzes the transfer of a glucosyl group to flavonol, forming the initial glucoside. Subsequently, it facilitates the addition of a second glucosyl moiety, yielding the final bis-glucoside product. Our production process leverages advanced eukaryotic expression systems to enhance the functional purity and activity of the Glycosyltransferase, ensuring high yields of this complex glucoside.

Fig. 1. Comparative 3D structure of Cp3GT and its N-terminal truncated form Cp3GTΔ80. (OA Literature) Fig.1 Three-dimensional structural comparison of Cp3GT with an N-terminal truncated variant Cp3GT80.¹

Service Scope

Are you currently facing high inclusion body formation in E. coli, inconsistent glycosylation patterns, or recombinant tags interfering with the active sites of your plant-derived enzymes? Our custom flavonol 3-O-glucosyltransferase production service helps you obtain high-purity, functionally validated enzymes for specialized flavonoid research through our proprietary eukaryotic expression platforms and advanced kinetic profiling. By utilizing optimized P. pastoris systems and site-specific tag engineering, Creative Biolabs eliminates the common bottlenecks in plant enzyme synthesis, accelerating your path from genetic discovery to commercial-grade biocatalysis.

Production Pipeline for Flavonol 3-O-Glucosyltransferase

Gene Optimization & Synthesis
We perform codon optimization specifically for eukaryotic hosts (Yeast/Insect) to avoid rare codon usage that stalls translation in traditional systems.

Expression Host Screening
We test multiple conditions in P. pastoris systems to ensure the flavonol 3-O-glucosyltransferase is post-translationally modified and secreted in a soluble, active form.

Advanced Purification
Utilizing multi-step chromatography, we achieve >95% purity. We offer specialized tag removal services to ensure that recombinant anchors do not interfere with the PSPG-box or catalytic residues.

Kinetic Profiling
We validate the enzyme against a panel of flavonols to determine Km and Vmax values, ensuring the batch meets your precise research requirements.

Final Documentation & Delivery
You receive the purified enzyme alongside a certificate of analysis detailing activity units and purity.

Fig. 2. Synthesis pipeline of flavonol 3-O-glucosyltransferase. (Creative Biolabs AI) Fig.2 Flavonol 3-O-glucosyltransferase manufacturing process.

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Service Features

Platform Superiority
Published research and real-world data solidly back up the excellence of our yeast-based expression systems. These systems are adept at handling intricate folding processes that frequently lead to inclusion body formation in bacterial hosts. By sidestepping this common pitfall, our yeast-based approach guarantees enzymes of superior quality and reliability, giving you an edge in your research and production endeavors.

Precision Kinetics
We harness the power of advanced technology to offer highly sensitive profiling of sugar-donor preferences. This enables us to gain a deep and precise understanding of enzyme kinetics, empowering you with detailed and actionable insights that drive your projects forward. Our precision kinetics approach ensures that you can optimize enzyme performance with confidence.

Scale Flexibility
Our production capabilities are highly versatile, catering to a broad spectrum of needs. Whether you require microgram quantities for crystallography studies or gram-scale amounts for pilot biotransformation projects, we possess the flexibility to meet your demands. Our scalable production solutions are designed to adapt to your changing requirements, ensuring seamless integration into your workflow.

Custom Enzyme Development
We specialize in providing bespoke enzyme development services, collaborating closely with you to design and engineer enzymes that match your unique specifications. Our team of seasoned experts employs state-of-the-art techniques to craft enzymes with heightened activity, robust stability, or unparalleled specificity. With our custom enzyme development services, you can unlock new possibilities and push the boundaries of what's achievable.

Customer Reviews

Tag-Free Design
"Using the custom flavonol 3-O-glucosyltransferase in our research has facilitated our understanding of flavonol-specific glycosylation. The tag-free version provided a 20% boost in activity compared to our in-house His-tagged constructs, enabling more precise kinetic studies and reducing downstream purification complexity."- 2 months ago, Dr. Alen** S.

Accelerated Timelines
"The transition from E. coli to their platform for our project was a game-changer. We obtained 50x more soluble enzyme, allowing us to complete our structural determination six months ahead of schedule and with higher-resolution data. The team's expertise in host system optimization was instrumental in overcoming our prior solubility bottlenecks."- 4 months ago, Prof. Ma** K.

Applications

Premium Agriculture & Enhanced Fruit Aesthetics

Through strategic enzyme engineering, we can finely tune the regulation of flavonol 3-O-glucosyltransferase. This precise control over these enzymes enables the targeted accumulation of anthocyanins in high-value fruit varieties.

Nutraceutical Innovation & Bioactive Fortification

Our advanced production techniques yield flavonol 3-O-glucosyltransferase of exceptional purity. This high-purity enzyme facilitates the large-scale biotransformation of flavonoids into more soluble and stable glycosides. Such transformations boost the therapeutic efficacy of bioactive compounds like quercetin and kaempferol, thereby elevating the health benefits of dietary supplements.

Unraveling Plant Defense Mechanisms

By engineering flavonol 3-O-glucosyltransferase with tailored substrate specificities, we empower researchers to delve deeper into the intricate ways plants employ flavonol glycosylation as a defense strategy. This knowledge sheds light on how plants combat abiotic stressors and viral pathogens, paving the way for the development of novel crop protection methods.

Structural Biology & Crystallography Excellence

Our enzyme production process is designed to meet the exacting standards of structural biology research. We produce enzymes with high purity, free from extraneous tags, and featuring proper post-translational modifications. This clean enzyme production approach is ideal for X-ray diffraction and NMR studies, enabling detailed characterization of plant glycosyltransferases.

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FAQs

Q: Why should I choose Pichia expression over E. coli for my flavonol 3-O-glucosyltransferase?

A: Plant glycosyltransferases often require eukaryotic folding machinery. Pichia prevents the formation of inclusion bodies common in E. coli, resulting in higher specific activity and purity.

Q: Can you produce the enzyme without any purification tags?

A: Yes. We offer tag-free production or enzymatic tag removal because research shows tags can sometimes interfere with the enzyme's active site.

Q: What is the typical lead time for a custom flavonol 3-O-glucosyltransferase project?

A: Standard production takes 6-8 weeks, depending on the complexity of the sequence and the required purification steps.

Q: Do you provide kinetic data with the delivered enzyme?

A: Every batch comes with a certificate of analysis that includes purity (SDS-PAGE) and specific activity data against your target flavonol substrate.

Q: Are these enzymes suitable for industrial biocatalysis?

A: Absolutely. We offer scale-up services for clients moving from lab-scale research to pilot-scale bioproduction.

Extended Services

Custom Flavonol 3-O-β-D-Glucosyl-(1→2)-β-D-Glucoside Production Service

We offer tailored production services for flavonol 3-O-β-D-glucosyl-(1→2)-β-D-glucoside, utilizing advanced enzyme engineering techniques to ensure high purity, yield, and customization according to your specific research or industrial needs.

Bioactive Carbohydrates Screening Services

Our bioactive carbohydrates screening services employ cutting-edge technologies to identify and evaluate carbohydrates with potential therapeutic or nutritional benefits, providing you with valuable insights for product development and innovation.

Start a Conversation

Creative Biolabs delivers industry-leading, end-to-end solutions for custom flavonol 3-O-glucosyltransferase production, seamlessly transforming genetic sequences into high-purity, functionally validated enzymes. Our integrated workflow spans gene synthesis, heterologous expression optimization, and rigorous analytical testing to ensure batch consistency and performance reliability. For technical specifications, project timelines, or collaborative opportunities, contact our expert team today.

Reference

Birchfield, Aaron S., and Cecilia A. McIntosh. "Expression and purification of Cp3GT: Structural analysis and modeling of a key plant flavonol-3-O glucosyltransferase from Citrus paradisi." BioTech 13.1 (2024): 4. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/biotech13010004

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