GlycoErase™ HEK293T B3GNT4 Knockout Cell Line (CAT#: GLJF-0425-JF502)

Description	To advance the study of poly-N-acetyllactosamine chain biosynthesis, we are pleased to present a B3GNT4 knockout HEK293T cell line. This cell line, deficient in the B3GNT4-encoded UDP-GlcNAc:betaGal beta-1,3-O-acetylglucosaminyltransferase 4, provides a unique opportunity to investigate the specific role of this enzyme in the biosynthetic pathway. By utilizing HEK293T cells, a platform known for its robust support of gene function and regulation studies, researchers can gain a deeper understanding of the intricacies of poly-N-acetyllactosamine chain formation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	B3GNT4
Full Name	UDP-GlcNAc:BetaGal Beta-1,3-O-acetylglucosaminyltransferase 4
Alternative Name	B3GN-T4, beta3Gn-T4
Location	12q24.31
Gene ID	79369
Summary	Involved in the biosynthesis of poly-N-acetyllactosamine chains, utilizing lacto-N-neotetraose as a preferred substrate.