GlycoErase™ HEK293T STT3A Knockout Cell Line

We are pleased to offer an STT3A knockout HEK293T cell line for researchers investigating N-linked glycosylation. This cell line, deficient in the STT3A-encoded STT3 oligosaccharyltransferase complex catalytic subunit A, provides a unique tool to study the OST complex's function in transferring glycan chains to nascent proteins in the endoplasmic reticulum. This model is particularly valuable for exploring the impact of STT3A disruption on N-linked glycosylation and its downstream effects on protein folding, stability, and function within the context of HEK293T cells, a platform widely used for gene function and regulation studies.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

STT3A

STT3 oligosaccharyltransferase complex catalytic subunit A

11q24.2

Catalytic subunit of the N-oligosaccharyltransferase (OST) complex, responsible for transferring glycan chains to asparagine residues of target proteins in the endoplasmic reticulum.