GlycoFlux™ Human SIGLEC12 Overexpression Jurkat Cell Line

GlycoFlux™ human SIGLEC12 overexpression Jurkat cell line is a stable T-cell based model engineered to overexpress SIGLEC12, an immunoglobulin-like lectin receptor that transmits inhibitory signals through ITIM and SLAM-like motifs. Activation leads to SHP1/2 recruitment, dampening macrophage-driven immune responses. With multiple transcript variants arising from alternative splicing, SIGLEC12 plays a complex role in shaping immune tolerance and host-pathogen interactions. This glycoengineered Jurkat model enables precise investigation of glycan-receptor biology, T-cell cross-talk with inhibitory lectins, and the role of SIGLEC12 in immune checkpoint pathways.

Overexpression Cell Lines

Human

Suspension-growing

1:3~1:5

Jurkat cell line

T-cell acute lymphoblastic leukemia (T-ALL)

T Lymphocyte

T-cell acute lymphoblastic leukemia (T-ALL)

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & RPMI

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC12

Sialic Acid Binding Ig Like Lectin 12

S2V, SLG, SIGLECL1, Siglec-XII, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the SIGLEC3-like subfamily, characterized by extracellular V-set immunoglobulin-like domains, followed by two C2-set domains, along with cytoplasmic ITIM and SLAM-like motifs. Upon tyrosine phosphorylation, the protein recruits SHP1 and SHP2, functioning as an inhibitory receptor in macrophage signaling regulation. The gene is located on 19q13.4, with multiple transcript variants due to alternative splicing.