GlycoFlux™ Human SIGLEC12 Overexpression HepG2 Cell Line

GlycoFlux™ human SIGLEC12 overexpression HepG2 cell line is a stable glycoengineered cell line designed to express SIGLEC12, a member of the SIGLEC3-like subfamily characterized by an extracellular V-set Ig-like domain, two C2-set Ig-like domains, and cytoplasmic ITIM and SLAM-like motifs. Upon tyrosine phosphorylation, SIGLEC12 recruits SHP1/2 phosphatases to mediate inhibitory signaling in macrophages, suppressing immune activation. The gene is located within a SIGLEC cluster at 19q13.4 and generates multiple isoforms through alternative splicing. This system provides a robust model to study sialic acid-dependent inhibitory signaling, tumor immune evasion, and glycosylation-related modulation of innate immunity.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

DMEM & FBS & Glutamine

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC12

Sialic Acid Binding Ig Like Lectin 12

S2V, SLG, SIGLECL1, Siglec-XII, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the SIGLEC3-like subfamily, characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set domains, and cytoplasmic motifs ITIM and SLAM-like. Upon tyrosine phosphorylation, the protein recruits SHP1 and SHP2, and functions as an inhibitory receptor to negatively regulate macrophage signaling. This gene is clustered with other SIGLEC3-like genes on 19q13.4, and alternative splicing results in multiple transcript variants.