GlycoFlux™ Human SIGLEC12 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF860)

Description	GlycoFlux™ human SIGLEC12 overexpression HepG2 cell line is a stable glycoengineered cell line designed to express SIGLEC12, a member of the SIGLEC3-like subfamily characterized by an extracellular V-set Ig-like domain, two C2-set Ig-like domains, and cytoplasmic ITIM and SLAM-like motifs. Upon tyrosine phosphorylation, SIGLEC12 recruits SHP1/2 phosphatases to mediate inhibitory signaling in macrophages, suppressing immune activation. The gene is located within a SIGLEC cluster at 19q13.4 and generates multiple isoforms through alternative splicing. This system provides a robust model to study sialic acid-dependent inhibitory signaling, tumor immune evasion, and glycosylation-related modulation of innate immunity.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC12
Full Name	Sialic Acid Binding Ig Like Lectin 12
Alternative Name	S2V, SLG, SIGLECL1, Siglec-XII, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	89858
Summary	This gene encodes a member of the SIGLEC3-like subfamily, characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set domains, and cytoplasmic motifs ITIM and SLAM-like. Upon tyrosine phosphorylation, the protein recruits SHP1 and SHP2, and functions as an inhibitory receptor to negatively regulate macrophage signaling. This gene is clustered with other SIGLEC3-like genes on 19q13.4, and alternative splicing results in multiple transcript variants.