GlycoFlux™ Lenti-Human PYGL Viral Particle

GlycoFlux™ Lenti-PYGL Viral Particle provides PYGL, the liver glycogen phosphorylase converting glycogen to glucose-1-phosphate; phosphorylation at Ser15 activates the enzyme, and mutations cause GSD VI (Hers disease). This replication-defective vector is intended for generating stable mammalian cell lines for hepatic glucose-output modeling, hormonal/allosteric control assays, and variant rescue with routine selection or fluorescence enrichment.

Recombinant Lentivirus

PYGL

Human

EF1a

Lentivirus

HEK293T

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Glycogen Phosphorylase L

GSD6

14q22.1

The product of this gene is a homodimeric enzyme that releases glucose-1-phosphate from liver glycogen by cleaving alpha-1,4-glucosidic bonds. Conversion from inactive phosphorylase B to active phosphorylase A occurs via phosphorylation at serine 15. Additional regulation is mediated by allosteric effectors and hormones. Mutations cause glycogen storage disease type VI (Hers disease), marked by moderate hypoglycemia, mild ketosis, growth delay, and hepatomegaly. Multiple isoforms are generated by alternative splicing.