GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit, Micro-method

The GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of ascorbic acid oxidase (AAO) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Vitamin Series

AAO

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of ascorbic acid oxidase (AAO) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Ascorbic Acid Oxidase

Ascorbic Acid Oxidase; AAO; Ascorbic Acid Oxidoreductase

As a pivotal biomolecule, ascorbic acid oxidase (AAO) plays a central role in complex physiological networks. It mediates crucial electron transfer processes, neutralizing reactive species or facilitating biochemical oxidations. Moreover, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Designed for comprehensive functional analysis of ascorbic acid oxidase (AAO) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.