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GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX347

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit offers an advanced quantitative methodology for evaluating ascorbic acid oxidase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Vitamin Series
Target
AAO
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target ascorbic acid oxidase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of AAO levels.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Ascorbic Acid Oxidase
Alternate Names
Ascorbic Acid Oxidase; AAO; Ascorbic Acid Oxidoreductase
Application Notes
Background
Functioning at the core of various biological processes, ascorbic acid oxidase (AAO) is a key subject of biochemical analysis. This biocatalyst heavily influences cellular redox balance by driving specific oxidation or reduction events. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Designed for comprehensive functional analysis of ascorbic acid oxidase (AAO) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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