GlycoSens™ Ascorbate Oxidase (AAO) Activity Assay Kit

The GlycoSens™ Ascorbate Oxidase (AAO) Activity Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target ascorbate oxidase activity in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

AAO

Substrate Mix A; Substrate Mix B; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of ascorbate oxidase activity. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect AAO activity in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Ascorbate Oxidase

Ascorbate Oxidase Activity; AAO; Ascorbate Oxidase Activity Standard; Ascorbate Oxidase Activity Reagent

Ascorbate oxidase (AAO) activity is recognized as a fundamental component in biological systems. It mediates crucial electron transfer processes, neutralizing reactive species or facilitating biochemical oxidations. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Ideal for investigating ascorbate oxidase activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Stable Signal Output; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.