GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit

The GlycoSens™ Ascorbic Acid Oxidase (AAO) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target ascorbic acid oxidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Ascorbate-Glutathione Cycle Series

AAO

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of ascorbic acid oxidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect AAO content in the sample.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Ascorbic Acid Oxidase

Ascorbic Acid Oxidase; AAO; Ascorbic Acid Oxidoreductase

The presence and activity of ascorbic acid oxidase (AAO) are closely linked to overall organismal health. It plays a central role in mitigating oxidative stress and managing intracellular reactive oxygen intermediates. In addition, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Tailored for accurate quantification of ascorbic acid oxidase (AAO) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Linear Dynamic Range; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.