GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit, Micro-method

The GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit offers an advanced quantitative methodology for evaluating dihydroflavonol 4-reductase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Anthocyanin Synthesis Series

DFR

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The system measures chromatic product formation derived from target dihydroflavonol 4-reductase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of DFR levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Dihydroflavonol 4-Reductase

Dihydroflavonol 4-Reductase; DFR

Dihydroflavonol 4-reductase (DFR) is an integral element required for robust cellular function and metabolism. It plays a central role in mitigating oxidative stress and managing intracellular reactive oxygen intermediates. Furthermore, assessing this component offers a reliable parameter for agricultural research or biological studies.

Designed for comprehensive functional analysis of dihydroflavonol 4-reductase (DFR) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.