GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit, Spectrophotometry

The GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target dihydroflavonol 4-reductase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Anthocyanin Synthesis Series

DFR

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

20 μL - 100 μL

The assay relies on the distinct spectroscopic optical characteristics of dihydroflavonol 4-reductase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect DFR content in the sample.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Dihydroflavonol 4-Reductase

Dihydroflavonol 4-Reductase; DFR

The presence and activity of dihydroflavonol 4-reductase (DFR) are closely linked to overall organismal health. It accelerates targeted redox reactions that are fundamental for stress response and metabolic adaptation. Furthermore, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Widely applicable for tracking dihydroflavonol 4-reductase (DFR) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.