GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit

The GlycoSens™ Dihydroflavonol 4-Reductase (DFR) Assay Kit Assay Kit utilizes a highly stable detection system tailored for accurate profiling of dihydroflavonol 4-reductase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

DFR

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, cellular lysates, other biological samples

10 μL - 50 μL

Target dihydroflavonol 4-reductase (DFR) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target DFR.

Within 2 hours

500 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Dihydroflavonol 4-Reductase

Dihydroflavonol 4-Reductase; DFR

In the context of biological research, dihydroflavonol 4-reductase (DFR) has emerged as a molecule of high interest. It accelerates targeted redox reactions that are fundamental for stress response and metabolic adaptation. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Widely applicable for tracking dihydroflavonol 4-reductase (DFR) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.