GlycoSens™ Glucosinoside Assay Kit, Micro-method

The GlycoSens™ Glucosinoside Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target glucosinoside in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Carbohydrate Metabolism Series

Glucosinoside

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of glucosinoside. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect glucosinoside content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glucosinoside

Glucosinoside

Glucosinoside is recognized as a fundamental component in biological systems. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Consequently, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Essential for monitoring glucosinoside level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.