GlycoSens™ Hexokinase (HK) Assay Kit

The GlycoSens™ Hexokinase (HK) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of hexokinase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

HK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

50 μL

Target hexokinase (HK) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target HK.

Within 1 hour

340 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hexokinase

Hexokinase; HK; Hexokinase Standard; Hexokinase Reagent

Research highlights hexokinase (HK) as a significant participant in key metabolic and regulatory cascades. This enzyme operates as a critical regulatory switch, modulating the activity of downstream signaling proteins. Consequently, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Tailored for accurate quantification of hexokinase (HK) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

High Specificity; Broad Sample Compatibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.