GlycoSens™ Hexokinase (HK) Assay Kit, Micro-method

The GlycoSens™ Hexokinase (HK) Assay Kit offers an advanced quantitative methodology for evaluating hexokinase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Glycolysis Series

HK

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The system measures chromatic product formation derived from target hexokinase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of HK levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hexokinase

Hexokinase; HK

The presence and activity of hexokinase (HK) are closely linked to overall organismal health. It drives ATP-dependent phosphorylation events that govern cellular growth, division, and metabolism. Consequently, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring hexokinase (HK) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.