GlycoSens™ Hexokinase (HK) Assay Kit, Spectrophotometry

The GlycoSens™ Hexokinase (HK) Assay Kit offers an advanced quantitative methodology for evaluating hexokinase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Glycolysis Series

HK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

The system measures chromatic product formation derived from target hexokinase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of HK levels.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hexokinase

Hexokinase; HK

Hexokinase (HK) is recognized as a fundamental component in biological systems. By phosphorylating key molecular targets, it orchestrates complex intracellular communication networks. As a result, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Tailored for accurate quantification of hexokinase (HK) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.