GlycoSens™ Hydrogen Peroxide (H₂O₂) Assay Kit, Micro-method

The GlycoSens™ Hydrogen Peroxide (H₂O₂) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of hydrogen peroxide (H₂O₂) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

H₂O₂

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Cell lysates, tissue homogenates

20 μL - 100 μL

Quantification of hydrogen peroxide (H₂O₂) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

415 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydrogen Peroxide

Hydrogen Peroxide; H₂O₂

Hydrogen peroxide (H₂O₂) is an integral element required for robust cellular function and metabolism. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Notably, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Widely applicable for tracking hydrogen peroxide (H₂O₂) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.