GlycoSens™ Hydrogen Peroxide (H₂O₂) Assay Kit, Spectrophotometry

The GlycoSens™ Hydrogen Peroxide (H₂O₂) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of hydrogen peroxide (H₂O₂) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

H₂O₂

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Serum, plasma, tissues, cell culture supernatants

5 μL - 20 μL

Quantification of hydrogen peroxide (H₂O₂) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

415 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydrogen Peroxide

Hydrogen Peroxide; H₂O₂

As a pivotal biomolecule, hydrogen peroxide (H₂O₂) plays a central role in complex physiological networks. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Furthermore, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Optimized for high-throughput profiling of hydrogen peroxide (H₂O₂) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.