GlycoSens™ Hydrogen/Potassium-Transporting Adenosine Triphosphatase (H⁺/K⁺-ATPase) Assay Kit

The GlycoSens™ Hydrogen/Potassium-Transporting Adenosine Triphosphatase (H⁺/K⁺-ATPase) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target hydrogen/potassium-transporting adenosine triphosphatase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

H⁺/K⁺-ATPase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of hydrogen/potassium-transporting adenosine triphosphatase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect H⁺/K⁺-ATPase content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydrogen/Potassium-Transporting Adenosine Triphosphatase

Hydrogen/Potassium-Transporting Adenosine Triphosphatase; H⁺/K⁺-ATPase; Hydrogen/Potassium-Transporting Adenosine Triphosphatase Standard; Hydrogen/Potassium-Transporting Adenosine Triphosphatase Reagent

Known for its specific biological characteristics, hydrogen/potassium-transporting adenosine triphosphatase (H⁺/K⁺-ATPase) provides deep insights into cellular dynamics. It plays a fundamental role in regulating intracellular ion concentrations and overall membrane potential. Furthermore, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Designed for comprehensive functional analysis of hydrogen/potassium-transporting adenosine triphosphatase (H⁺/K⁺-ATPase) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Stable Signal Output; Broad Sample Compatibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.