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GlycoSens™ β-Mannosidase (β-Man) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX1477

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ β-Mannosidase (β-Man) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target β-mannosidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Other Series
Target
β-Man
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
20 μL - 100 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of β-mannosidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect β-Man content in the sample.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
β-Mannosidase
Alternate Names
β-Mannosidase; β-Man
Application Notes
Background
Functioning at the core of various biological processes, β-mannosidase (β-man) is a key subject of biochemical analysis. It effectively accelerates specific biochemical transformations, ensuring the rapid progression of metabolic pathways. Consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Specifically engineered for evaluating β-mannosidase (β-Man) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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