GlycoSens™ β-Mannosidase (β-Man) Assay Kit

The GlycoSens™ β-Mannosidase (β-Man) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target β-mannosidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Cell Wall Metabolism Series

β-Man

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

The assay relies on the distinct spectroscopic optical characteristics of β-mannosidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect β-Man content in the sample.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

β-Mannosidase

β-Mannosidase; β-Man

Known for its specific biological characteristics, β-mannosidase (β-man) provides deep insights into cellular dynamics. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. As a result, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Optimized for high-throughput profiling of β-mannosidase (β-Man) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Linear Dynamic Range; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.