GlycoSens™ β-Mannosidase (β-Man) Assay Kit, Microplate Method

The GlycoSens™ β-Mannosidase (β-Man) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of β-mannosidase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

β-Man

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

Target β-mannosidase (β-Man) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target β-Man.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

β-Mannosidase

β-Mannosidase; β-Man

The presence and activity of β-mannosidase (β-man) are closely linked to overall organismal health. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Notably, assessing this component offers a reliable parameter for agricultural research or biological studies.

Optimized for high-throughput profiling of β-mannosidase (β-Man) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.